bd clontech ab microarray analysis workbook Search Results


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TaKaRa mouse anti mouse p21 mab
Figure 4. Induction of p53 target genes in T1 and T2 tumor derived cells treated with 5-fluorouracil (5-FU) and the presence of p19ARF transcript in MPC wild type (wt) and their tumor-derived counterparts. A, Western blot analysis of <t>p21</t> induction in MPCEWS-FLI-1-T1/T2-derived cells treated with 50 Ag/mL 5-fluorouracil or solvent (DMSO) for 16 hours, confirming the preservation of a functional p53 pathway. B, induction of p53 target genes in the same cells as in (A), as assessed by cDNA microarray analysis. C, semiquantitative RT-PCR analysis confirming the presence of a p19ARF transcript in wild-type MPC and its induction in the same cells overexpressing c-myc. No induction by c-myc is observed in p19ARF/ MPCs. D, presence of p19ARF in MPCEWS-FLI-1-T1/T2 tumor–derived cells, as assessed by RT-PCR.
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Becton Dickinson nylon microarray
<t>Microarray</t> analysis of RNA samples from pretreatment tumours and 24 h post-CDDP-treatment (6 mg kg −1 i.p.). Each gene is spotted in duplicate on the array. Genes increased/decreased >2.5-fold by CDDP-treatment are indicated. For further details see Materials and Methods.
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Becton Dickinson total human 1.2 cdna microarray
<t>Microarray</t> analysis of RNA samples from pretreatment tumours and 24 h post-CDDP-treatment (6 mg kg −1 i.p.). Each gene is spotted in duplicate on the array. Genes increased/decreased >2.5-fold by CDDP-treatment are indicated. For further details see Materials and Methods.
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Becton Dickinson antibody microarray
<t>Microarray</t> analysis of RNA samples from pretreatment tumours and 24 h post-CDDP-treatment (6 mg kg −1 i.p.). Each gene is spotted in duplicate on the array. Genes increased/decreased >2.5-fold by CDDP-treatment are indicated. For further details see Materials and Methods.
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Becton Dickinson ab microarray analysis workbook
<t>Microarray</t> analysis of RNA samples from pretreatment tumours and 24 h post-CDDP-treatment (6 mg kg −1 i.p.). Each gene is spotted in duplicate on the array. Genes increased/decreased >2.5-fold by CDDP-treatment are indicated. For further details see Materials and Methods.
Ab Microarray Analysis Workbook, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson atlas human cdna array-1
<t>Microarray</t> analysis of RNA samples from pretreatment tumours and 24 h post-CDDP-treatment (6 mg kg −1 i.p.). Each gene is spotted in duplicate on the array. Genes increased/decreased >2.5-fold by CDDP-treatment are indicated. For further details see Materials and Methods.
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Becton Dickinson atlas rat 3.8 microarrays
<t>Microarray</t> analysis of RNA samples from pretreatment tumours and 24 h post-CDDP-treatment (6 mg kg −1 i.p.). Each gene is spotted in duplicate on the array. Genes increased/decreased >2.5-fold by CDDP-treatment are indicated. For further details see Materials and Methods.
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Becton Dickinson atlas™ plastic microarrays
<t>Microarray</t> analysis of RNA samples from pretreatment tumours and 24 h post-CDDP-treatment (6 mg kg −1 i.p.). Each gene is spotted in duplicate on the array. Genes increased/decreased >2.5-fold by CDDP-treatment are indicated. For further details see Materials and Methods.
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86
TaKaRa data analysis antibody microarrays
<t>Microarray</t> analysis of RNA samples from pretreatment tumours and 24 h post-CDDP-treatment (6 mg kg −1 i.p.). Each gene is spotted in duplicate on the array. Genes increased/decreased >2.5-fold by CDDP-treatment are indicated. For further details see Materials and Methods.
Data Analysis Antibody Microarrays, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TaKaRa antibody microarray
<t>Microarray</t> analysis of RNA samples from pretreatment tumours and 24 h post-CDDP-treatment (6 mg kg −1 i.p.). Each gene is spotted in duplicate on the array. Genes increased/decreased >2.5-fold by CDDP-treatment are indicated. For further details see Materials and Methods.
Antibody Microarray, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TaKaRa random primer mix
<t>Microarray</t> analysis of RNA samples from pretreatment tumours and 24 h post-CDDP-treatment (6 mg kg −1 i.p.). Each gene is spotted in duplicate on the array. Genes increased/decreased >2.5-fold by CDDP-treatment are indicated. For further details see Materials and Methods.
Random Primer Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TaKaRa bd clontech ab microarray analysis workbook
<t>Microarray</t> analysis of RNA samples from pretreatment tumours and 24 h post-CDDP-treatment (6 mg kg −1 i.p.). Each gene is spotted in duplicate on the array. Genes increased/decreased >2.5-fold by CDDP-treatment are indicated. For further details see Materials and Methods.
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Image Search Results


Figure 4. Induction of p53 target genes in T1 and T2 tumor derived cells treated with 5-fluorouracil (5-FU) and the presence of p19ARF transcript in MPC wild type (wt) and their tumor-derived counterparts. A, Western blot analysis of p21 induction in MPCEWS-FLI-1-T1/T2-derived cells treated with 50 Ag/mL 5-fluorouracil or solvent (DMSO) for 16 hours, confirming the preservation of a functional p53 pathway. B, induction of p53 target genes in the same cells as in (A), as assessed by cDNA microarray analysis. C, semiquantitative RT-PCR analysis confirming the presence of a p19ARF transcript in wild-type MPC and its induction in the same cells overexpressing c-myc. No induction by c-myc is observed in p19ARF/ MPCs. D, presence of p19ARF in MPCEWS-FLI-1-T1/T2 tumor–derived cells, as assessed by RT-PCR.

Journal: Cancer Research

Article Title: Development of Ewing's Sarcoma from Primary Bone Marrow–Derived Mesenchymal Progenitor Cells

doi: 10.1158/0008-5472.can-05-1696

Figure Lengend Snippet: Figure 4. Induction of p53 target genes in T1 and T2 tumor derived cells treated with 5-fluorouracil (5-FU) and the presence of p19ARF transcript in MPC wild type (wt) and their tumor-derived counterparts. A, Western blot analysis of p21 induction in MPCEWS-FLI-1-T1/T2-derived cells treated with 50 Ag/mL 5-fluorouracil or solvent (DMSO) for 16 hours, confirming the preservation of a functional p53 pathway. B, induction of p53 target genes in the same cells as in (A), as assessed by cDNA microarray analysis. C, semiquantitative RT-PCR analysis confirming the presence of a p19ARF transcript in wild-type MPC and its induction in the same cells overexpressing c-myc. No induction by c-myc is observed in p19ARF/ MPCs. D, presence of p19ARF in MPCEWS-FLI-1-T1/T2 tumor–derived cells, as assessed by RT-PCR.

Article Snippet: Primary mouse anti-V5 epitope mAb (Invitrogen), mouse anti-ErbB2 mAb (Chemicon, Temecula, CA), mouse anti-mouse p21 mAb (BD Biosciences Clontech), hamster anti-mouse Bcl2 mAb (BD PharMingen), polyclonal rabbit anti-mouse Igf1 receptor a (Cell Signaling, Beverly, MA), and Histone H3 (Abcam, Cambridge, United Kingdom) antibodies were used.

Techniques: Derivative Assay, Western Blot, Solvent, Preserving, Functional Assay, Microarray, Reverse Transcription Polymerase Chain Reaction

Microarray analysis of RNA samples from pretreatment tumours and 24 h post-CDDP-treatment (6 mg kg −1 i.p.). Each gene is spotted in duplicate on the array. Genes increased/decreased >2.5-fold by CDDP-treatment are indicated. For further details see Materials and Methods.

Journal: British Journal of Cancer

Article Title: Characterisation of molecular events following cisplatin treatment of two curable ovarian cancer models: contrasting role for p53 induction and apoptosis in vivo

doi: 10.1038/sj.bjc.6602167

Figure Lengend Snippet: Microarray analysis of RNA samples from pretreatment tumours and 24 h post-CDDP-treatment (6 mg kg −1 i.p.). Each gene is spotted in duplicate on the array. Genes increased/decreased >2.5-fold by CDDP-treatment are indicated. For further details see Materials and Methods.

Article Snippet: The expression levels of 256 genes whose products are involved in the regulation of cell death and the cell cycle were profiled using a commercially available nylon microarray (BD Biosciences Clontech, Palo Alto, CA, USA).

Techniques: Microarray